基因的全化学组装
Total chemical assembly of genes.
作者信息
Shabarova Z A, Sokolova N I, Merenkova I N
机构信息
Chemistry Department, Moscow State University, USSR.
出版信息
Nucleic Acids Symp Ser. 1990(22):133-4.
PMID:2101898
Abstract
An artificial gene comprising 183 base pairs has been assembled by template-directed condensation of 35- to 53-membered oligodeoxyribonucleotides with cyanogen bromide as a condensing agent. The reaction is complete within several minutes at 0 degree C in buffer. The resulting gene was cloned into M13mp11 and sequenced using the Sanger sequencing procedure.
摘要
通过以溴化氰作为缩合剂，对35至53个成员的寡脱氧核糖核苷酸进行模板导向缩合，组装出了一个包含183个碱基对的人工基因。该反应在0摄氏度的缓冲液中几分钟内即可完成。所得基因被克隆到M13mp11中，并使用桑格测序程序进行测序。
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