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Constraction of a subtracted cDNA library using oligo(dT)-latex.

作者信息

Hara E, Kato T, Nakada S, Nakajima T, Tsurui H, Oda K

机构信息

Department of Applied Biological Science, Science University of Tokyo, Chiba, Japan.

出版信息

Nucleic Acids Symp Ser. 1990(22):29-30.

PMID:2101904
Abstract

An efficient method for constraction of subtracted cDNA library was developed using oligo(dT 30. Latex and PCR. This method improved the chances for identifying cDNA clones corresponding to scarce class of mRNA that is expressed differentially during cellular growth and differentiation.

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