用寡聚（dT）30-乳胶快速高效地纯化聚腺苷酸（poly(A)）-mRNA。
A rapid and efficient purification of poly(A)-mRNA by oligo(dT)30-Latex.
作者信息
Kuribayashi K, Hikata M, Hiraoka O, Miyamoto C, Furuichi Y
机构信息
Japan Synthetic Rubber Co., Ltd., Tokyo Research Laboratory, Kawasaki, Japan.
出版信息
Nucleic Acids Symp Ser. 1988(19):61-4.
Latex particles were covalently linked to the 5'-proximal region of oligo(dT)30. The resultant oligo(dT)30-Latex was tested for its hybridizability to poly(A) containing mRNA. Several advantages were noted as compared to the conventional oligo(dT)30-cellulose column chromatography; (1) a highly efficient (approximately 95%) hybridization occurs in a short reaction period (10min), (2) more than 95% of poly(A) mRNA can be recovered from oligo(dT)30-Latex by a simple heating followed by brief centrifugation, (3) multiple samples can be handled simultaneously and moreover, (4) the poly(A)-mRNA on the oligo(dT)30-Latex can be directly transcribed by AMV reverse transcriptase to form the cDNA. These properties of oligo(dT)30-Latex promise an excellent reagent for nucleic acid technology.
将乳胶颗粒共价连接到寡聚（dT）30的5'-近端区域。测试所得的寡聚（dT）30-乳胶与含poly（A）的mRNA的杂交能力。与传统的寡聚（dT）30-纤维素柱层析相比，具有几个优点：（1）在短反应时间（10分钟）内发生高效（约95%）杂交，（2）通过简单加热然后短暂离心，可从寡聚（dT）30-乳胶中回收超过95%的poly（A）mRNA，（3）可同时处理多个样品，此外，（4）寡聚（dT）30-乳胶上的poly（A）-mRNA可被禽成髓细胞瘤病毒（AMV）逆转录酶直接转录形成cDNA。寡聚（dT）30-乳胶的这些特性有望成为核酸技术的优良试剂。
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