用于结构研究的寡核糖核苷酸的化学合成。
Chemical synthesis of oligoribonucleotides for structural studies.
作者信息
Sasaki T, Iwai S, Tsuda S, Hikichi K, Ohtsuka E
机构信息
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
出版信息
Nucleic Acids Symp Ser. 1990(22):43-4.
PMID:2101911
Abstract
Large-scale synthesis of oligoribonucleotides has been performed successfully on a solid support by the phosphoramidite approach using levulinyl and tetrahydrofuranyl protection for the 5'- and 2'-hydroxyl groups respectively. A hexamer containing inosine and four fragments of a hammerhead-type ribozyme have been synthesized on a 10 mumol scale for structural studies by NMR spectroscopy and X-ray crystallography.
摘要
通过磷酰胺方法，分别使用乙酰丙酸酯和四氢呋喃基对5'-和2'-羟基进行保护，已成功在固相载体上大规模合成寡核糖核苷酸。已以10微摩尔规模合成了一种含有次黄嘌呤的六聚体和锤头型核酶的四个片段，用于通过核磁共振光谱和X射线晶体学进行结构研究。
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