Silani V, Pizzuti A, Donato M F, Falini A, Bassani R, Strada O, Causarano R I, Mariani D, Villani R M, Scarlato G
Centro Dino Ferrari, Universitá di Milano.
Boll Ist Sieroter Milan. 1990;69(1):309-13.
The paper reviews some conceptual and methodological aspects of the tissue culture models which, during the past three decades, demonstrated a remarkable mimicry of many important structures and functions of the mammalian Central Nervous System (CNS) and related peripheral sensory and motor elements. Emphasis is placed on an original human neuronal tissue culture model obtained from selective CNS areas. The different cell types were identified and the neurotrophic interactions preliminary characterized. Neuropathological findings suggest hypothesis that can be fully tested using in vitro human models of affected cerebral specific areas.
本文回顾了组织培养模型的一些概念和方法学方面的内容，在过去三十年中，这些模型展现出对哺乳动物中枢神经系统（CNS）以及相关外周感觉和运动元件的许多重要结构和功能的显著模拟。重点是一种从选择性中枢神经系统区域获得的原始人类神经元组织培养模型。已识别出不同的细胞类型，并初步表征了神经营养相互作用。神经病理学发现提出了一些假说，这些假说可以使用受影响脑特定区域的体外人类模型进行充分验证。
