Pulliam L, Berens M E, Rosenblum M L
Department of Laboratory Medicine, Veterans Administration Medical Center, San Francisco, CA 94121.
J Neurosci Res. 1988 Oct-Dec;21(2-4):521-30. doi: 10.1002/jnr.490210243.
A new in vitro model of normal human brain has been developed in which fetal human brain cells form three-dimensional aggregates that can be maintained for up to 60 days in culture. Cells appear fully differentiated at the time of initiation in culture; the predominant cells identified were astrocytes, neurons, and oligodendrocytes with myelin, with occasional ependymal cells and macrophages. The specific arrangement and numbers of neural cells within aggregates differed among brain specimens. Cell kinetics studies detected DNA synthesis throughout the culture interval. Aggregates cocultured with a human malignant glioma cell line (U251-MG) were progressively invaded by tumor cells. In aggregates infected with human cytomegalovirus (CMV), intracellular viral replication and morphologic changes characteristic of human brain infection with this pathogen were seen. This model of brain aggregates should prove valuable for multidisciplinary studies in human neurobiology, particularly in the fields of developmental neurobiology, neuro-oncogenesis, tumor cell invasion, and species-specific viral infection of the central nervous system.
一种新的正常人脑体外模型已被开发出来，在该模型中，人胎儿脑细胞形成三维聚集体，可在培养中维持长达60天。细胞在培养开始时似乎已完全分化；鉴定出的主要细胞是星形胶质细胞、神经元和有髓鞘的少突胶质细胞，偶尔还有室管膜细胞和巨噬细胞。不同脑标本中聚集体内神经细胞的具体排列和数量有所不同。细胞动力学研究在整个培养期间都检测到了DNA合成。与人类恶性胶质瘤细胞系（U251-MG）共培养的聚集体被肿瘤细胞逐渐侵袭。在感染人巨细胞病毒（CMV）的聚集体中，可见细胞内病毒复制以及该病原体感染人脑时的形态学变化。这种脑聚集体模型对于人类神经生物学的多学科研究应该是有价值的，特别是在发育神经生物学、神经肿瘤发生、肿瘤细胞侵袭以及中枢神经系统的种属特异性病毒感染等领域。
