Salwa J
Laboratory of Tumor Immunology, Institute of Immunology and Experimental Therapy, Polish Academy of Science, Wrocław.
Arch Immunol Ther Exp (Warsz). 1990;38(3-4):267-74.
The ability of peritoneal exudate cells (PECs) and their adherent (APECs) and nonadherent (NAPECs) fractions to enhance RPC-5 plasmacytoma growth in vitro was studied. The capability of these cells to bind RPC-5 cells and influence of the binding on cytolysis tumor cells by activated with C. parvum macrophages was also determined. The effector cells were harvested from mice injected i.p. with pristane, thioglicollate medium or C. parvum or from intact mice. The effect of supernatants from the in vitro cultured PECs, APECs or NAPECs on growth RPC-5 cells were also tested. It was found that the RPC-5 plasmacytoma growth was enhanced only by cells obtained from mice treated with pristane, or by supernatants from cultured PECs and APECs derived from pristane treated mice. The adherent cells from pristane treated mice were able to bind tumor cells. The tumor cells preexposed to adherent cells from pristane stimulated mice were resistant to lysis by activated with C. parvum macrophages.
研究了腹膜渗出细胞（PEC）及其贴壁部分（APEC）和非贴壁部分（NAPEC）在体外增强RPC-5浆细胞瘤生长的能力。还测定了这些细胞结合RPC-5细胞的能力以及这种结合对经微小隐孢子虫激活的巨噬细胞溶解肿瘤细胞的影响。效应细胞从小鼠腹腔注射降植烷、巯基乙酸盐培养基或微小隐孢子虫后收获，或从完整小鼠中收获。还测试了体外培养的PEC、APEC或NAPEC的上清液对RPC-5细胞生长的影响。发现只有从用降植烷处理的小鼠获得的细胞，或从用降植烷处理的小鼠来源的培养PEC和APEC的上清液才能增强RPC-5浆细胞瘤的生长。来自用降植烷处理的小鼠的贴壁细胞能够结合肿瘤细胞。预先暴露于来自经降植烷刺激的小鼠的贴壁细胞的肿瘤细胞对经微小隐孢子虫激活的巨噬细胞的裂解具有抗性。
