Błach-Olszewska Z, Juszczyk M, Zaczyńska E
Laboratory of Virology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Worcław.
Arch Immunol Ther Exp (Warsz). 1993;41(1):63-6.
Freshly isolated resident peritoneal cells (RPC) of three mouse strains: BALB/c, NZB and C3H, release spontaneously small amounts of tumor necrosis factor (TNF) when cultured in vitro. RPC of BALB/c mice produced higher TNF levels than the cells isolated from two other strains (BALB/c > NZB > C3H). Lipopolysaccharide (LPS) potentiated the TNF production. Kinetics of the TNF production depended on temperature of incubation of the cells. At 37 degrees C TNF was produced earlier (with a peak at 7 h) than at 26 degrees C (a peak at 20 h). Adherent cell fraction of RPC produced TNF at 26 degrees C as well as at 37 degrees C. Nonadherent cells released TNF only at 37 degrees C. The neutralization assay with media containing TNF and polyclonal rabbit anti-mouse TNF-alpha serum revealed that in every case, independently on the incubation temperature and cell fraction origin, only TNF-alpha was detected. Antibodies against human monoclonal or polyclonal TNF-alpha did not neutralize mouse TNF.
三种小鼠品系（BALB/c、NZB和C3H）的新鲜分离的驻留腹膜细胞（RPC）在体外培养时会自发释放少量肿瘤坏死因子（TNF）。BALB/c小鼠的RPC产生的TNF水平高于从其他两种品系分离的细胞（BALB/c＞NZB＞C3H）。脂多糖（LPS）增强了TNF的产生。TNF产生的动力学取决于细胞孵育的温度。在37℃时，TNF产生得更早（在7小时达到峰值），而在26℃时（在20小时达到峰值）则较晚。RPC的贴壁细胞部分在26℃和37℃时都产生TNF。非贴壁细胞仅在37℃时释放TNF。用含有TNF的培养基和多克隆兔抗小鼠TNF-α血清进行的中和试验表明，在每种情况下，无论孵育温度和细胞部分来源如何，仅检测到TNF-α。抗人单克隆或多克隆TNF-α的抗体不能中和小鼠TNF。
