多态单分子的 FRET 效率分布。
FRET efficiency distributions of multistate single molecules.
机构信息
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
出版信息
J Phys Chem B. 2010 Nov 25;114(46):15221-6. doi: 10.1021/jp105359z. Epub 2010 Oct 28.
Abstract
A simple analytic theory is developed to describe FRET efficiency histograms constructed from a photon trajectory generated by a molecule with multiple conformational states. The histograms are approximated by a sum of Gaussians with the parameters explicitly determined by the FRET efficiencies of the states and the rates of the transitions between the states. The theory, which has been tested against exact histograms for two conformational states and simulated data for three and four conformational states, accurately describes how the peaks in the histograms collapse as the bin time or the transition rates increase.
摘要
本文发展了一种简单的分析理论，用于描述由具有多个构象态的分子产生的光子轨迹构建的 FRET 效率直方图。直方图通过具有明确由状态的 FRET 效率和状态之间的跃迁速率确定的参数的高斯函数的和来近似。该理论已经针对两个构象态的精确直方图和三个和四个构象态的模拟数据进行了测试，准确地描述了随着 bin 时间或跃迁速率的增加，直方图中的峰如何坍塌。
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