Institute of Polymer Science, School of Chemistry and Chemical Engineering, BME Center, State Key Laboratory of Optoelectronic Materials and Technologies, DSAPM Lab and PCFM Lab, Sun Yat-sen University, Guangzhou 510275, China.
Biomacromolecules. 2010 Dec 13;11(12):3395-405. doi: 10.1021/bm100902d. Epub 2010 Oct 28.
An acidic tea polysaccharide (ALTPS), isolated from green tea ( Camellia sinensis ), was characterized as a hyperbranched glycoprotein containing the acidic heteropolysaccharide chains and the protein residues from the results of UV-vis, FTIR, one- and two-dimensional NMR, GC, GC-MS, and amino acid analyses. Solution properties of ALTPS were investigated by static and dynamic light scattering analyses and viscometry. The results indicated that the viscosity behavior of ALTPS exhibited a typical polyelectrolyte effect in distilled water, which may be avoided by adding salts. The low intrinsic viscosity of ALTPS in the solutions (8-15 mL/g) is attributed to its hyperbranched structure. By application of the polymer solution theory, it was revealed that ALTPS was present in a sphere-like conformation in the solutions as a result of the hyperbranched structure. The TEM image further confirmed that ALTPS existed in a spherical conformation in aqueous NaCl solution. Glucose was absorbed by ALTPS, which may be one of blood glucose lowering mechanisms of tea polysaccharides.
从绿茶（Camellia sinensis）中分离出的酸性茶多糖（ALTPS）被鉴定为一种高度支化的糖蛋白，其结果来自于 UV-vis、FTIR、一维和二维 NMR、GC、GC-MS 和氨基酸分析，含有酸性杂多糖链和蛋白质残基。通过静态和动态光散射分析和粘度法研究了 ALTPS 的溶液性质。结果表明，ALTPS 在蒸馏水中的粘度行为表现出典型的聚电解质效应，可以通过添加盐来避免。在溶液中（8-15 mL/g），ALTPS 的低特性粘度归因于其高度支化的结构。通过聚合物溶液理论的应用，揭示了由于高度支化的结构，ALTPS 在溶液中呈球型构象存在。TEM 图像进一步证实，ALTPS 在 NaCl 水溶液中呈球形构象存在。葡萄糖被 ALTPS 吸收，这可能是茶多糖降血糖机制之一。
