茶花多糖缀合物的纯化与特性研究。
Study on the purification and characterization of a polysaccharide conjugate from tea flowers.
机构信息
Institute of Food Engineering, College of Life & Environment Science, Shanghai Normal University, 100 Guilin Rd, Shanghai 200234, PR China.
出版信息
Int J Biol Macromol. 2010 Aug 1;47(2):266-70. doi: 10.1016/j.ijbiomac.2010.04.011. Epub 2010 Apr 27.
In this paper, the crude polysaccharides from the flowers of tea plant (Camellia sinensis) (TFPS) extracted with hot water were fractionated on a DEAE Sepharose FF chromatography to get TFPS1 with a yield of 18%. The properties and chemical compositions of TFPS1 were analyzed with GC, HPGPC, IC, IR methods and its morphology was observed with atomic force microscopy (AFM). The results showed that TFPS1 was a neutral glycoprotein conjugate with a molecular weight 500kDa. The alanine, threonine, glycine, valine, serine, histidine, glutamic acid, histidine and tyrosine were found in TFPS1 and the total content was 2.03%. TFPS1 was consisted of rhamnose, arabinose, mannose, glucose and galactose, with a mole ratio of 1.0:2.9:0.5:1.3:3.3. Sugar backbone of TFPS1 may consist of glucose and galactose, but branched chain may consist of arabinose, galactose and rhamnose. The IR spectrum of TFPS1 revealed the typical characteristics of polysaccharides and protein. TFPS1 was spherical particle structure with a diameter of 50-70nm.
本文采用热水浸提法从茶花（Camellia sinensis）花中提取粗多糖（TFPS），并通过 DEAE Sepharose FF 层析法对其进行分离，得到产率为 18%的 TFPS1。采用 GC、HPGPC、IC、IR 等方法分析 TFPS1 的性质和化学组成，并用原子力显微镜（AFM）观察其形态。结果表明，TFPS1 是一种中性糖蛋白缀合物，分子量为 500kDa。TFPS1 中含有丙氨酸、苏氨酸、甘氨酸、缬氨酸、丝氨酸、组氨酸、谷氨酸、组氨酸和酪氨酸，总含量为 2.03%。TFPS1 由鼠李糖、阿拉伯糖、甘露糖、葡萄糖和半乳糖组成，摩尔比为 1.0：2.9：0.5：1.3：3.3。TFPS1 的糖链可能由葡萄糖和半乳糖组成，但支链可能由阿拉伯糖、半乳糖和鼠李糖组成。TFPS1 的红外光谱显示出多糖和蛋白质的典型特征。TFPS1 为 50-70nm 直径的球形颗粒结构。
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