Biosurfaces Unit, CIC biomaGUNE, Paseo Miramon 182, 20009 Donostia-San Sebastian, Spain.
Anal Chem. 2010 Nov 15;82(22):9275-81. doi: 10.1021/ac102495q. Epub 2010 Oct 28.
We report a new and label-free method to detect and characterize the clustering of membrane-bound proteins and, by extension, the lateral segregation of nanosized particles adsorbed to planar surfaces in liquid environment. The method exploits the contrast between two different mass and surface sensitive detection methods, quartz crystal microbalance with dissipation monitoring and ellipsometry. The time-resolved correlation of both techniques provides insight into subtle changes in the clustering state of surface-bound molecules that is not accessible with either technique alone. A theoretical model can provide quantitative predictions about the size of surface-bound clusters.
我们报告了一种新的、无需标记的方法，用于检测和表征膜结合蛋白的聚集，并扩展到检测和表征在液体环境中吸附到平面表面的纳米级颗粒的侧向分离。该方法利用了两种不同的质量和表面敏感检测方法（石英晶体微天平与耗散监测和椭圆偏振术）之间的对比。两种技术的时间分辨相关性提供了对表面结合分子聚集状态的细微变化的深入了解，而这些变化是单独使用任何一种技术都无法获得的。一个理论模型可以对表面结合簇的大小提供定量预测。
