Marsilio S, Kleinschmidt S, Harder J, Nolte I, Hewicker-Trautwein M
Department of Pathology, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany.
Anat Histol Embryol. 2011 Feb;40(1):61-7. doi: 10.1111/j.1439-0264.2010.01039.x. Epub 2010 Oct 28.
In this study, CD3(+) T lymphocytes and IgA(+) , IgG(+) and IgM(+) plasma cells were quantified in the tunica mucosa of the intestinal tract of 12 pet cats without gastrointestinal diseases. The study included full-thickness biopsies of the duodenum, jejunum, ileum and colon. The distribution and quantification of CD3(+) T cells, IgA(+) , IgG(+) and IgM(+) plasma cells within the intestinal tunica mucosa was performed by using immunohistochemical methods and computer-aided morphometry. CD3(+) T cells were significantly prominent in the villi and their numbers increased from duodenum to ileum but decreased towards the colon. The predominant type of plasma cells was IgA(+) cells, followed by IgM(+) cells. The number of IgG(+) cells was generally low compared to the other plasma cell types investigated. The results of the vertical distribution showed that IgA(+) and IgM(+) plasma cells were most numerous in the lower crypt areas, whilst IgG(+) plasma cells accumulated in the upper crypt region with a decline towards the villi and the lower crypt areas of control cats. All types of plasma cells showed a general decline from the duodenum towards the caudal sections of the intestinal tract regarding the horizontal distribution of plasma cells. This study provides a comprehensive overview on the vertical and horizontal distribution and the number of CD3(+) T cells and IgA(+) , IgG(+) and IgM(+) plasma cells in the intestinal tunica mucosa of pet cats.
在本研究中，对12只无胃肠道疾病的宠物猫肠道黏膜层中的CD3(+) T淋巴细胞以及IgA(+)、IgG(+)和IgM(+)浆细胞进行了定量分析。该研究包括十二指肠、空肠、回肠和结肠的全层活检。采用免疫组化方法和计算机辅助形态测量法，对肠道黏膜层内CD3(+) T细胞、IgA(+)、IgG(+)和IgM(+)浆细胞进行分布和定量分析。CD3(+) T细胞在绒毛中显著突出，其数量从十二指肠到回肠增加，但向结肠减少。浆细胞的主要类型是IgA(+)细胞，其次是IgM(+)细胞。与所研究的其他浆细胞类型相比，IgG(+)细胞数量通常较少。垂直分布结果显示，IgA(+)和IgM(+)浆细胞在隐窝下部区域数量最多，而IgG(+)浆细胞在隐窝上部区域积聚，在对照猫的绒毛和隐窝下部区域数量减少。就浆细胞的水平分布而言，所有类型的浆细胞从十二指肠到肠道尾部均呈总体减少趋势。本研究全面概述了宠物猫肠道黏膜层中CD3(+) T细胞以及IgA(+)、IgG(+)和IgM(+)浆细胞的垂直和水平分布及数量。
