Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei, China.
Am J Reprod Immunol. 2011 May;65(5):503-11. doi: 10.1111/j.1600-0897.2010.00921.x. Epub 2010 Oct 4.
What is the relationship between Th17 cells and unexplained recurrent spontaneous abortion (URSA)?
CD4(+) interleukin (IL)-17A(+) T (Th17) cells and CD4(+) CD25(bright) Foxp3(+) regulatory T (Treg) cells in peripheral blood were analyzed by flow cytometry; IL-17 concentrations in cell culture supernatants were quantitatively determined by enzyme-linked immunosorbent assay; and IL-17A positive cells in decidua tissues were measured by immunohistochemistry.
The proportion of Th17 cells and IL-17A concentrations was both significantly higher in patients with URSA than in normal early pregnant (NEP) and non-pregnant (NP) patients, Treg frequencies were significantly lower in patients with URSA than in NEP patients, and the ratio of Th17 to Treg was significantly higher in the URSA group than in the other two. Additionally, the percentage of IL-17A cells in deciduas was significantly higher in patients with URSA than in NEP patients.
Human Th17 cells may play a major role in rejecting conceptus antigens and therefore may be harmful to the maintenance of pregnancy. The data also suggest that Treg cells are beneficial to pregnancy. There may exist a balance of Th17/Treg in normal pregnancy.
Th17 细胞与不明原因复发性自然流产（URSA）之间有何关系？
采用流式细胞术分析外周血中 CD4(+)白细胞介素（IL）-17A(+)T（Th17）细胞和 CD4(+)CD25(bright)Foxp3(+)调节性 T（Treg）细胞；用酶联免疫吸附试验定量测定细胞培养上清液中的 IL-17 浓度；用免疫组织化学法测定蜕膜组织中 IL-17A 阳性细胞。
URSA 患者外周血 Th17 细胞比例和 IL-17A 浓度均显著高于正常早孕（NEP）和非妊娠（NP）患者，URSA 患者 Treg 频率显著低于 NEP 患者，URSA 组 Th17/Treg 比值显著高于其他两组。此外，URSA 患者蜕膜中 IL-17A 细胞的百分比显著高于 NEP 患者。
人类 Th17 细胞可能在排斥胚胎抗原方面发挥重要作用，因此可能对妊娠的维持有害。该数据还表明 Treg 细胞有利于妊娠。在正常妊娠中可能存在 Th17/Treg 的平衡。
