Division of Gastroenterology, Tohoku University Graduate School of Medicine, Sendai City, Miyagi, Japan.
Cancer Sci. 2011 Jan;102(1):150-6. doi: 10.1111/j.1349-7006.2010.01757.x. Epub 2010 Oct 13.
The incidence and mortality of cholangiocarcinoma are increasing despite improvements in the diagnostic method. Since the sensitivity of brushing cytology for cholangiocarcinoma is not satisfactory, a novel diagnostic marker needs to be established. A recent report has suggested upregulation of the calcium-binding protein S100P in cholangiocarcinoma. The expression status of S100P in normal bile duct and cholangiocarcinoma tissues was assessed by immunohistochemistry. The expression levels of S100P mRNA in the brushing cytology samples during endoscopic retrograde cholangiopancreatography (ERCP) from benign biliary strictures and cholangiocarcinoma were assessed by real-time reverse transcription-polymerase chain reaction (RT-PCR). The sensitivity and specificity of each diagnostic strategy was compared. S100P was frequently expressed in the cholangiocarcinoma tissues, but not in the normal bile duct. The brushing cytology samples from the cholangiocarcinoma cases revealed higher expression levels of S100P compared with the benign biliary strictures. The relative expression level of S100P could determine the cholangiocarcinoma at higher sensitivity than classical cytology, and the combination of the S100P expression level and cytology yielded a sensitivity of 90.0%, with a specificity of 92.0%. Calcium-binding protein S100P is a novel marker of cholangiocarcinoma. Detecting the S100P expression levels in brushing cytology samples has a diagnostic value, which will be helpful for better diagnosis of cholangiocarcinoma.
尽管诊断方法有所改进，胆管癌的发病率和死亡率仍在上升。由于刷检细胞学对胆管癌的敏感性并不令人满意，因此需要建立新的诊断标志物。最近的一份报告表明，钙结合蛋白 S100P 在胆管癌中上调。通过免疫组织化学评估 S100P 在正常胆管和胆管癌组织中的表达状态。通过实时逆转录-聚合酶链反应（RT-PCR）评估经内镜逆行胰胆管造影（ERCP）时良性胆道狭窄和胆管癌刷检细胞学样本中 S100P mRNA 的表达水平。比较了每种诊断策略的敏感性和特异性。S100P 在胆管癌组织中频繁表达，但在正常胆管中不表达。与良性胆道狭窄相比，胆管癌病例的刷检细胞学样本中 S100P 的表达水平更高。S100P 的相对表达水平比经典细胞学更能确定胆管癌，S100P 表达水平和细胞学的组合具有 90.0%的敏感性和 92.0%的特异性。钙结合蛋白 S100P 是胆管癌的一种新型标志物。检测刷检细胞学样本中 S100P 的表达水平具有诊断价值，有助于更好地诊断胆管癌。
