Division of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry, Sendai, Japan.
Oral Dis. 2011 May;17(4):379-86. doi: 10.1111/j.1601-0825.2010.01761.x. Epub 2010 Oct 28.
The study was designed to investigate the development of roots and periodontal tissues after allogenic tooth transplantation between rat littermates by micro-computed tomography (micro-CT) and histology.
The upper right second molars in 2-week-old rats were extracted and immediately transplanted into the upper right first molar socket of rat littermates under anesthesia. The upper left second molars in 2-week-old recipient rats were used as a control. The rats were fixed and tissues analyzed at 0, 4, 8, or 12 weeks after transplantation. Root development of seven rats in each group was analyzed quantitatively using micro-CT. Periodontal tissue formation was examined qualitatively by histologic methods.
Roots developed after allogenic transplantation, but they were significantly shorter than control roots. The number of roots varied from one to four in transplanted teeth, while it was consistently four in control teeth. Periodontal tissue formation in transplanted teeth was equivalent to that of the control teeth.
Allogenic transplantation between rat littermates permits root development and periodontal tissue formation.
本研究旨在通过 micro-CT 和组织学方法研究同种异体牙移植后大鼠亲代个体间的牙根和牙周组织的发育。
在麻醉下，将 2 周龄大鼠的右上第二磨牙立即移植到其亲代大鼠右上第一磨牙牙槽窝中。将 2 周龄受者大鼠的左上第二磨牙作为对照。在移植后 0、4、8 或 12 周时将大鼠固定并分析组织。使用 micro-CT 对每组中的 7 只大鼠的牙根发育进行定量分析。通过组织学方法定性检查牙周组织的形成。
同种异体移植后牙根发育，但明显短于对照牙根。移植牙的牙根数量从 1 到 4 不等，而对照牙的牙根数量始终为 4。移植牙的牙周组织形成与对照牙相当。
大鼠亲代个体间的同种异体移植可促进牙根发育和牙周组织形成。
