National Research Center for Wildlife Born Diseases, Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China.
Vaccine. 2010 Dec 16;29(2):323-8. doi: 10.1016/j.vaccine.2010.10.034. Epub 2010 Oct 26.
The aim of this work was to evaluate efficiency as well as the type of immune response, Th1 or Th2, induced by multivalent DNA vaccinations in C57BL/6 interleukin-12p40 (IL-12p40) knockout (KO) mice. A recombinant pVAX-15-23 plasmid DNA was constructed by inserting surface glycoprotein (cp15- and p23)-encoding DNA into the pVAX1 expression vector. Various parameters including antibody and cytokine responses, proliferation assay and oocyst shedding were used to evaluate the type of immune response and the level of protection against challenge infection. Obtained results indicated that plasmid pVAX-15-23 induced strong protective immune response against C. parvum characterized by dominance of IgG2a, high level of INF-γ and lower level of the oocysts shedding after challenge infection. Moreover, co-immunization with the multivalent DNA and pMEM12R plasmid encoding IL-12 can further enhance these responses compared with the multivalent DNA alone. The obtained results suggest that multivalent pVAX-15-23 DNA vaccine may be a candidate as a generic approach to C. parvum immunization applicable to clinical practice.
本研究旨在评估多价 DNA 疫苗在 C57BL/6 白细胞介素-12p40（IL-12p40）基因敲除（KO）小鼠中诱导的效率和免疫反应类型，Th1 或 Th2。通过将表面糖蛋白（cp15 和 p23）编码 DNA 插入 pVAX1 表达载体，构建了重组 pVAX-15-23 质粒 DNA。使用抗体和细胞因子反应、增殖试验和卵囊脱落等各种参数来评估免疫反应类型和对挑战感染的保护水平。获得的结果表明，质粒 pVAX-15-23 诱导了针对 C. parvum 的强烈保护性免疫反应，其特征是 IgG2a 占优势、高水平的 INF-γ 和挑战感染后卵囊脱落水平较低。此外，与单独使用多价 DNA 相比，与编码 IL-12 的 pMEM12R 质粒共同免疫可以进一步增强这些反应。研究结果表明，多价 pVAX-15-23 DNA 疫苗可能是一种针对 C. parvum 免疫的通用方法，适用于临床实践。
