Knaippe F
Institute of Microbiology, Federal University of Rio de Janeiro, Brasil.
Microsc Electron Biol Celular. 1990;14(1):35-43.
Analysis of the attachment basis of Giardia lamblia 1/Portland strain trophozoites to confluent MDCK (Madin Darby Canine Kidney) cell monolayers and type I collagen films, demonstrated, by the use of light and electron microscopy, that the ventral disk as well as the ventrolateral border are the structures involved in the substrate adhesion. Furthermore, we noticed that trophozoite attachment is more effective to collagen than to the apical surface of the epithelium. We described, for the first time, a rounded shape structure evaginated from the naked area that seems to be the ventral disk contact site between the trophozoite and the substrate. This structure has also been observed when the trophozoite is attached to coverslip glass or among trophozoites in close contact.
对蓝氏贾第鞭毛虫1/波特兰株滋养体附着于汇合的MDCK（麦迪逊-达比犬肾）细胞单层和I型胶原膜的附着基础进行分析，通过光学显微镜和电子显微镜观察发现，腹侧盘以及腹外侧边缘是参与底物黏附的结构。此外，我们注意到滋养体对胶原的附着比对上皮细胞顶端表面的附着更有效。我们首次描述了一种从裸露区域外翻出的圆形结构，它似乎是滋养体与底物之间腹侧盘的接触位点。当滋养体附着于盖玻片或紧密接触的滋养体之间时，也观察到了这种结构。
