Campanati Loraine, Holloschi Andreas, Troster Helmut, Spring Herbert, de Souza Wanderley, Monteiro-Leal Luiz Henrique
Universidade do Estado do Rio de Janeiro, Dept. de Histologia e Embriologia, Laboratório de Microscopia e Processamento de Imagens, Maracaná Rio de Janeiro, Brasil.
Cell Motil Cytoskeleton. 2002 Apr;51(4):213-24. doi: 10.1002/cm.10026.
Video-microscopy in combination with digital image processing was used to analyze dynamic processes associated to the life cycle of Giardia lamblia trophozoites. These parasites swim and attach to the epithelial cells, producing the disease known as Giardiasis. Giardia is a multiflagellar cell, presenting 4 pairs of flagella. With the use of analogue and digital tools, we observed that in cells attached to glass slides only 2 of the 4 pairs present active beating (wave propagation). The frequency observed was 17-18 Hz to the anterior and 8-11 Hz to the ventral flagella. These data resulted from several hours of recording using both analogue video and high-speed digital camera. The caudal pair did not show active beating patterns and the same holds true for the posterior one. In this latter pair, oscillations were observed, but they were always associated to the transit of the wave produced by the ventral pair. The analysis performed with free moving cells showed that during its forward dislocation, Giardia lamblia presented either a lateral rocking or a complete rotational (tumbling) movement around its longitudinal axis. A dislocation of the caudal region of the cell both in the lateral and dorso-ventral direction was observed. This movement was completely independent from the flagellar beating and it is likely to be produced by a microtubular complex located in the caudal portion of the cell. The adhesion process of Giardia lamblia was also followed by video-microscopy and the data showed that the ventral disk had an active participation in this process.
视频显微镜结合数字图像处理技术被用于分析与蓝氏贾第鞭毛虫滋养体生命周期相关的动态过程。这些寄生虫游动并附着于上皮细胞，引发名为贾第虫病的疾病。贾第虫是一种多鞭毛细胞，有4对鞭毛。通过使用模拟和数字工具，我们观察到附着在载玻片上的细胞中，4对鞭毛中只有2对呈现活跃摆动（波传播）。观察到前侧鞭毛的频率为17 - 18赫兹，腹侧鞭毛的频率为8 - 11赫兹。这些数据来自使用模拟视频和高速数码相机进行的数小时记录。尾侧的一对鞭毛未显示活跃的摆动模式，后侧的一对也是如此。在后侧这对鞭毛中，观察到有振荡，但它们总是与腹侧鞭毛产生的波的传递相关。对自由游动细胞进行的分析表明，蓝氏贾第鞭毛虫在向前移动时，呈现出围绕其纵轴的侧向摇摆或完全旋转（翻滚）运动。观察到细胞尾侧区域在侧向和背腹方向都有位移。这种运动完全独立于鞭毛的摆动，很可能是由位于细胞尾侧部分的微管复合体产生的。蓝氏贾第鞭毛虫的黏附过程也通过视频显微镜进行了跟踪，数据显示腹侧盘在这个过程中起到了积极作用。
