Department of Chemistry and Institute of Basic Science, Chonnam National University, Gwangju 500-757, Republic of Korea.
Biosens Bioelectron. 2011 Jan 15;26(5):2287-91. doi: 10.1016/j.bios.2010.09.053. Epub 2010 Oct 7.
This paper introduces the use of multi walled carbon nanotubes (MWCNTs) with palladium (Pd) nanoparticles in the electrocatalytic reduction of hydrogen peroxide (H(2)O(2)). We have developed and characterized a biosensor for H(2)O(2) based on Nafion(®) coated MWCNTs-Pd nanoparticles on a glassy carbon electrode (GCE). The Nafion(®)/MWCNTs-Pd/GCE electrode was easily prepared in a rapid and simple procedure, and its application improves sensitive determination of H(2)O(2). Characterization of the MWCNTs-Pd nanoparticle film was performed with transmission electron microscopy (TEM), Raman, and X-ray photoelectron spectroscopy (XPS). Cyclic voltammetry (CV) and amperometry (at an applied potential of -0.2V) measurements were used to study and optimize performance of the resulting peroxide biosensor. The proposed H(2)O(2) biosensor exhibited a wide linear range from 1.0 μM to 10 mM and a low detection limit of 0.3 μM (S/N=3), with a fast response time within 10s. Therefore, this biosensor could be a good candidate for H(2)O(2) analysis.
本文介绍了多壁碳纳米管（MWCNTs）与钯（Pd）纳米粒子在过氧化氢（H₂O₂）电催化还原中的应用。我们开发并表征了一种基于 Nafion（®）涂覆的 MWCNTs-Pd 纳米粒子在玻碳电极（GCE）上的 H₂O₂生物传感器。Nafion（®）/MWCNTs-Pd/GCE 电极可通过快速简便的程序轻松制备，其应用可提高 H₂O₂的灵敏测定。通过透射电子显微镜（TEM）、拉曼和 X 射线光电子能谱（XPS）对 MWCNTs-Pd 纳米粒子薄膜进行了表征。采用循环伏安法（CV）和安培法（在施加的-0.2V 电位下）测量来研究和优化所得过氧化物生物传感器的性能。所提出的 H₂O₂生物传感器在 1.0 μM 至 10 mM 的宽线性范围内表现出良好的性能，检测限低至 0.3 μM（S/N=3），响应时间在 10s 内。因此，该生物传感器可能是 H₂O₂分析的良好候选者。
