GIRO Technological Centre, Centre IRTA-UPC, Barcelona, Spain.
Bioresour Technol. 2011 Feb;102(3):2219-27. doi: 10.1016/j.biortech.2010.09.121. Epub 2010 Oct 8.
Fresh pig/cattle slaughterhouse waste mixtures, with different lipid-protein ratios, were characterized and their anaerobic biodegradability assessed in batch tests. The resultant methane potentials were high (270-300 L(CH4) kg(-1)(COD)) making them interesting substrates for the anaerobic digestion process. However, when increasing substrate concentrations in consecutive batch tests, up to 15 g(COD) kg(-1), a clear inhibitory process was monitored. Despite the reported severe inhibition, related to lipid content, the system was able to recover activity and successfully degrade the substrate. Furthermore, 16SrRNA gene-based DGGE results showed an enrichment of specialized microbial populations, such as β-oxidizing/proteolitic bacteria (Syntrophomonas sp., Coprothermobacter sp. and Anaerobaculum sp.), and syntrophic methanogens (Methanosarcina sp.). Consequently, the lipid concentration of substrate and the structure of the microbial community are the main limiting factors for a successful anaerobic treatment of fresh slaughterhouse waste.
新鲜猪/牛屠宰场废物混合物，具有不同的脂-蛋白比，其特征在于分批测试中评估其厌氧生物降解性。所得的甲烷潜力很高（270-300 L（CH4）kg（-1）（COD）），使它们成为厌氧消化过程的有趣底物。然而，当在连续批次测试中增加底物浓度，高达 15 g（COD）kg（-1）时，监测到明显的抑制过程。尽管报道的严重抑制与脂质含量有关，但该系统能够恢复活性并成功降解底物。此外，基于 16SrRNA 基因的 DGGE 结果显示了专门微生物种群的富集，例如β-氧化/蛋白分解细菌（Syntrophomonas sp.，Coprothermobacter sp.和 Anaerobaculum sp.）和共营养产甲烷菌（Methanosarcina sp.）。因此，底物的脂质浓度和微生物群落的结构是新鲜屠宰废物成功进行厌氧处理的主要限制因素。
