AIDS Virus Research Unit, National Institute for Communicable Diseases (NHLS), Private Bag X4, Sandringham 2131, Johannesburg, South Africa.
School of Molecular and Cell Biology, University of the Witwatersrand, Johannesburg 2050, South Africa.
J Med Microbiol. 2011 Feb;60(Pt 2):180-183. doi: 10.1099/jmm.0.024463-0. Epub 2010 Oct 28.
Lactobacillus jensenii, Lactobacillus crispatus, Lactobacillus iners, Lactobacillus gasseri and Lactobacillus vaginalis were identified by 16S rRNA gene sequencing as the predominant culturable vaginal Lactobacillus species in a group of South African women, comprising 24, 22, 10, 10 and 9 %, respectively. A significant effect of vaginal discharge syndrome (VDS) and bacterial vaginosis (BV) on the distribution of predominant Lactobacillus species was observed. Whilst L. crispatus isolates were almost equally distributed between individuals with and without VDS and were not significantly reduced in women with BV versus normal microflora, L. jensenii isolates were significantly reduced in women with VDS (P=0.022) and reduced in women with BV versus normal microflora (P=0.053). Unlike L. crispatus, L. jensenii isolates were also significantly reduced in women with BV-associated VDS versus women without VDS and with normal microflora (P=0.051). In addition, lysogeny was commonly observed for L. crispatus, with 77 % of isolates yielding phage particles with contractile and non-contractile tails. Only 29 % of L. jensenii isolates yielded phage particles, and these were visible as tailless or podo-like particles.
16S rRNA 基因测序鉴定出詹氏乳杆菌、卷曲乳杆菌、惰性乳杆菌、加氏乳杆菌和阴道乳杆菌是南非一组女性阴道中主要可培养的阴道乳杆菌属，分别占 24%、22%、10%、10%和 9%。阴道分泌物综合征（VDS）和细菌性阴道病（BV）对主要乳杆菌属的分布有显著影响。虽然 L. crispatus 分离株在 VDS 患者和无 VDS 患者之间的分布几乎相等，且在 BV 患者与正常菌群相比并未显著减少，但 L. jensenii 分离株在 VDS 患者中显著减少（P=0.022），在 BV 患者与正常菌群相比也显著减少（P=0.053）。与 L. crispatus 不同，L. jensenii 分离株在 BV 相关 VDS 患者中也明显少于无 VDS 且正常菌群的患者（P=0.051）。此外，L. crispatus 普遍存在溶原性，77%的分离株产生具有收缩性和非收缩性尾部的噬菌体颗粒。只有 29%的 L. jensenii 分离株产生噬菌体颗粒，这些颗粒表现为无尾或类似足状的颗粒。
