Seminar on Environment, School of Life and Environmental Science, Azabu University, Japan.
Exp Anim. 2010;59(5):589-94. doi: 10.1538/expanim.59.589.
We investigated the effects of interleukin-6 (IL-6) and cytokine-induced neutrophil chemoattractant-1 (CINC-1) on α(2)-macroglobulin (α2M) production in rats. IL-6-rich and CINC-1-rich fractions were separated from serum obtained from rats 12 h after injection with turpentine oil using gel-chromatography. Sexual dimorphism was observed in the peak levels of α2M after injection of IL-6-, CINC-1-, or a mixture of IL-6-and-CINC-1-rich fractions. No significant differences in α2M levels were observed in males after injection with IL-6- or CINC-1-rich fractions and those injected with normal serum obtained from healthy rats (control). In contrast, serum levels of α2M, 6 to 120 h after injection of a mixture of IL-6- and CINC-1-rich fractions were significantly higher than in control rats. These results suggest that IL-6 and CINC-1 contribute to α2M production in rats only when IL-6 and CINC-1 act synergistically.
我们研究了白细胞介素-6（IL-6）和细胞因子诱导的中性粒细胞趋化因子-1（CINC-1）对大鼠α2-巨球蛋白（α2M）产生的影响。用凝胶层析法从油松节油注射后 12 小时大鼠血清中分离出富含 IL-6 和 CINC-1 的级分。在注射 IL-6、CINC-1 或富含 IL-6 和 CINC-1 的级分混合物后，大鼠的α2M 峰值水平表现出性别二态性。雄性大鼠注射富含 IL-6 或 CINC-1 的级分和注射来自健康大鼠（对照）的正常血清后，α2M 水平没有显著差异。相比之下，注射富含 IL-6 和 CINC-1 的级分混合物后 6 至 120 小时，血清中α2M 的水平明显高于对照组。这些结果表明，只有当 IL-6 和 CINC-1 协同作用时，它们才会促进大鼠的α2M 产生。
