Kuribayashi Takashi, Seita Tetsuro, Momotani Eiichi, Yamazaki Shunsuke, Hagimori Kohei, Yamamoto Shizuo
Graduate School of Environmental and Health Science, Azabu University, 1-17-71 Fuchinobe, Chou-ku, Sagamihara, Kanagawa, 252-5201, Japan.
Inflammation. 2015 Aug;38(4):1401-5. doi: 10.1007/s10753-015-0114-4.
The half-lives of typical acute phase proteins in rats and beagle dogs during acute inflammation were investigated. Acute inflammation was induced by injection of turpentine oil in rats and administration of indomethacin in beagle dogs. Serum concentrations of α2-macroglobulin (α2M) and C-reactive protein (CRP) were measured by enzyme-linked immunosorbent assay and α1-acid glycoprotein (AAG) was measured by single radial immunodiffusion. Half-life was calculated as 0.693/elimination rate constant (K). The mean half-lives in the terminal elimination phase of α2M and AAG were 68.1 and 164.8 h, respectively. The half-life of AAG was significantly longer than that of α2M. Mean half-lives in the terminal elimination phase of CRP and AAG were 161.9 and 304.4 h, respectively. The half-life of AAG was significantly longer than that of CRP in beagle dogs. No significant differences in the half-life of AAG were observed between rats and beagle dogs. Furthermore, serum concentrations in the terminal elimination phase could be simulated with the K data acquired in this study.
研究了大鼠和比格犬在急性炎症期间典型急性期蛋白的半衰期。通过给大鼠注射松节油和给比格犬施用吲哚美辛诱导急性炎症。采用酶联免疫吸附测定法测量血清中α2-巨球蛋白（α2M）和C反应蛋白（CRP）的浓度，采用单向放射免疫扩散法测量α1-酸性糖蛋白（AAG）的浓度。半衰期计算为0.693除以消除速率常数（K）。α2M和AAG在终末消除期的平均半衰期分别为68.1小时和164.8小时。AAG的半衰期明显长于α2M。CRP和AAG在终末消除期的平均半衰期分别为161.9小时和304.4小时。在比格犬中，AAG的半衰期明显长于CRP。在大鼠和比格犬之间未观察到AAG半衰期的显著差异。此外，终末消除期的血清浓度可以用本研究获得的K数据进行模拟。
