Ortiz-Zarragoitia Maren, Garmendia Larraitz, Barbero María Carmen, Serrano Teresa, Marigómez Ionan, Cajaraville Miren P
Biologia Zelularra eta Histologia Lab., Zoologia eta Biologia Zelularra Saila, Zientzia eta Teknologia Fakultatea, University of the Basque Country UPV/EHU, Sarriena z/g, 48940, Leioa, Basque Country, Spain.
J Environ Monit. 2011 Jan;13(1):84-94. doi: 10.1039/c0em00102c. Epub 2010 Oct 29.
The aim of this work was to assess possible effects of the Prestige oil spill on reproduction parameters of mussels along the Galician and Bay of Biscay coast. Studied endpoints included sex ratio, gonad histology and vitellogenin-like proteins using the alkali-labile phosphate (ALP) method. A high prevalence of haemocytic infiltration of follicles and severe oocyte atresia was found in most localities in April 2003. Spawning gonads were observed in most impacted populations in the same sampling. In April 2004 mature small sized follicles were observed. No histopathological changes were observed in April 2005 and 2006, except a high prevalence of necrotic gametes in 6 out of 22 localities in April 2006. Female ALP levels showed high interindividual variability in April 2004, which was reduced in April 2005 and 2006. No xenoestrogenic effects were observed in male mussels. Overall, gamete alterations were detected during 2003-2004 and a recovery trend was observed afterwards.
这项工作的目的是评估“威望号”油轮漏油事件对加利西亚和比斯开湾沿岸贻贝繁殖参数可能产生的影响。研究的终点指标包括性别比例、性腺组织学以及使用碱不稳定磷酸盐（ALP）法检测类卵黄蛋白原。2003年4月，在大多数地点发现卵泡血细胞浸润的高发生率和严重的卵母细胞闭锁。在同一次采样中，在大多数受影响的种群中观察到正在产卵的性腺。2004年4月观察到成熟的小型卵泡。2005年和2006年未观察到组织病理学变化，不过在2006年4月，22个地点中有6个出现了高发生率的坏死配子。2004年4月，雌性贻贝的ALP水平显示出较高的个体间变异性，2005年和2006年这种变异性有所降低。在雄性贻贝中未观察到外源性雌激素效应。总体而言，在2003 - 2004年期间检测到配子改变，之后观察到恢复趋势。
