Departamento de Microbiologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Paraná, Brazil.
Biol Res. 2010;43(2):233-41. Epub 2010 Sep 24.
The genus Phytomonas comprises trypanosomatids that can parasitize a broad range of plant species. These flagellates can cause diseases in some plant families with a wide geographic distribution, which can result in great economic losses. We have demonstrated previously that Phytomonas serpens 15T, a tomato trypanosomatid, shares antigens with Trypanosoma cruzi, the agent of human Chagas disease. Herein, two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to identify proteins of P. serpens 15T that are recognized by sera from patients with Chagas disease. After 2D-electrophoresis of whole-cell lysates, 31 peptides were selected and analyzed by tandem mass spectrometry. Twenty-eight polypeptides were identified, resulting in 22 different putative proteins. The identified proteins were classified into 8 groups according to biological process, most of which were clustered into a cellular metabolic process category. These results generated a collection of proteins that can provide a starting point to obtain insights into antigenic cross reactivity among trypanosomatids and to explore P. serpens antigens as candidates for vaccine and immunologic diagnosis studies.
植动物门包含可以寄生在广泛植物物种上的鞭毛原生动物。这些鞭毛虫可以引起一些植物科的疾病，具有广泛的地理分布，这可能导致巨大的经济损失。我们之前已经证明，番茄鞭毛虫 Phytomonas serpens 15T 与引起人类恰加斯病的寄生虫克氏锥虫 Trypanosoma cruzi 具有共同的抗原。在此，我们使用二维凝胶电泳（2-DE）和质谱（MS）来鉴定被恰加斯病患者血清识别的 P. serpens 15T 的蛋白质。在全细胞裂解物的 2D 电泳之后，选择了 31 个肽并通过串联质谱进行分析。鉴定出 28 个多肽，产生了 22 种不同的假定蛋白。根据生物过程对鉴定出的蛋白质进行分类，其中大多数被聚类到细胞代谢过程类别中。这些结果提供了一组蛋白质，这可以为研究鞭毛虫之间的抗原交叉反应以及探索 P. serpens 抗原作为疫苗和免疫诊断研究的候选物提供一个起点。
