Centro de Pesquisas Aggeu Magalhães - CPqAM/FIOCRUZ, Pernambuco, Brazil.
Phytother Res. 2010 Nov;24(11):1631-6. doi: 10.1002/ptr.3156.
Cramoll 1,4 is a lectin extracted from Cratylia mollis Mart. seeds that has shown antitumor and lymphocyte mitogenic activities in other studies. The aim of this work was to investigate, in vitro, the immunomodulatory activity of Cramoll 1,4 on experimental cultures of mice lymphocytes through cytotoxic assays, nitric oxide (NO) concentrations and IL-10 and IFN-γ production. Cramoll 1,4 did not show cytotoxic activity at 1-25 μg/mL concentrations, similar results were observed with concanavalin A (Con A) and phytohemagglutinin (PHA) lectins. The minimum production of IL-10 was observed in splenocytes cultivated with Con A, PHA and Cramoll 1,4 lectins. However, splenocytes treated with Cramoll 1,4 showed higher IFN-γ production in comparison with PHA and Con A (p < 0.05 for both). Production of NO was effectively suppressed in murine cells stimulated with the lectins and was only detected after 72 h for PHA in relation to non-stimulated lymphocytes (p < 0.05). Cramoll 1,4 was not toxic to murine lymphocytes, induced Th1 response through IFN-γ production and showed antiinflammatory activity through NO suppression. Therefore, Cramoll 1,4 can be considered a lectin with immunomodulatory activity.
Cramoll 1,4 是从克拉蒂利亚·莫利斯·马蒂种子中提取的一种凝集素，在其他研究中显示出抗肿瘤和淋巴细胞有丝分裂活性。本工作的目的是通过细胞毒性测定、一氧化氮（NO）浓度以及白细胞介素-10（IL-10）和干扰素-γ（IFN-γ）的产生，体外研究 Cramoll 1,4 对实验性小鼠淋巴细胞的免疫调节活性。在 1-25μg/mL 浓度下，Cramoll 1,4 没有显示细胞毒性活性，与刀豆球蛋白 A（Con A）和植物血球凝集素（PHA）凝集素的结果相似。在用 Con A、PHA 和 Cramoll 1,4 凝集素培养的脾细胞中观察到最低的 IL-10 产生。然而，与 PHA 和 Con A 相比，用 Cramoll 1,4 处理的脾细胞 IFN-γ的产生更高（两者均 p<0.05）。在用凝集素刺激的鼠细胞中，NO 的产生被有效抑制，并且仅在 72 小时后才检测到 PHA 与未刺激的淋巴细胞相比（p<0.05）。Cramoll 1,4 对小鼠淋巴细胞无毒，通过 IFN-γ产生诱导 Th1 反应，并通过抑制 NO 显示抗炎活性。因此，Cramoll 1,4 可被认为是一种具有免疫调节活性的凝集素。
