Departamento de Imunologia do Centro de Pesquisas Aggeu Magalhães - CPqAM/FIOCRUZ-UFPE, Pernambuco, Brazil.
Scand J Immunol. 2011 Feb;73(2):112-21. doi: 10.1111/j.1365-3083.2010.02490.x.
Cramoll 1,4 is a lectin with specific glucose/mannose binding, which is extracted from seeds of Cratylia mollis Mart. Many assays have shown the cytokine expression activity and anti-inflammatory profile of this lectin. The aim of this study was to evaluate the immunostimulatory response, in vitro, of splenocytes in mice previously inoculated, in vivo, with C. mollis (Cramoll 1,4) and Canavalia ensiformis (Con A) lectins. Results demonstrated higher proliferation indexes induced by Cramoll 1,4 than Con A lectin in relation to all experimental groups. Cramoll 1,4 and Con A also induced high levels of IL-2, IL-6, IFN-γ and nitric oxide production. Moreover, Cramoll 1,4 did not induce apoptosis and stimulated a significant number of cells in the S phase of the cell cycle. Results showed that Cramoll 1,4 lectin induces proliferative response and suggested that this lectin can be used as a mitogenic agent in immunostimulatory assays.
克拉莫尔 1,4 是一种具有特异性葡萄糖/甘露糖结合能力的凝集素，从山蚂蝗属植物种子中提取。许多研究表明，这种凝集素有细胞因子表达活性和抗炎作用。本研究旨在评估先前体内接种克拉莫尔 1,4（Cramoll 1,4）和刀豆球蛋白 A（Con A）凝集素的小鼠脾细胞的体外免疫刺激反应。结果表明，与所有实验组相比，克拉莫尔 1,4 诱导的增殖指数高于 Con A 凝集素。克拉莫尔 1,4 和 Con A 也诱导产生了高水平的 IL-2、IL-6、IFN-γ 和一氧化氮。此外，克拉莫尔 1,4 不会诱导细胞凋亡，而是刺激细胞周期 S 期的大量细胞。结果表明，克拉莫尔 1,4 凝集素诱导了增殖反应，提示该凝集素可用作免疫刺激试验中的有丝分裂原。
