Groupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval, 2420 Rue de la Terrasse, Québec City, QC, Canada.
Phytother Res. 2010 Nov;24(11):1687-92. doi: 10.1002/ptr.3195.
Plant extracts and/or secondary metabolites are receiving considerable attention as therapeutic agents for treating inflammatory diseases such as periodontitis, which affects the tooth supporting tissues. The aim of this study was to investigate the effect of a Grindelia robusta extract enriched in saponins and polyphenols on Aggregatibacter actinomycetemcomitans lipopolysaccharide (LPS)-induced inflammatory mediator (IL-6, TNF-a, RANTES, MCP-1, PGE(2) ) and matrix metalloproteinase (MMP-1, -3, -7, -8, -9, -13) secretion by macrophages. LPS induced a marked increase in the secretion of all inflammatory mediators and MMPs tested by macrophages, as determined by enzyme-linked immunosorbent assays. At non-cytotoxic concentrations, the G. robusta extract inhibited dose-dependently the secretion of IL-6, RANTES, MCP-1 and, to a lesser extent, PGE(2) and TNF-a. Such inhibition was also observed for MMP-1, -3, -7, -8, -9 and -13 secretion. This ability of G. robusta extract to reduce the LPS-induced secretion of inflammatory mediators and MMPs was associated with a reduction of nuclear factor-kappa B (NF-kB) p65 activation. The results suggest that G. robusta extract possesses an antiinflammatory therapeutic potential through its capacity to reduce the accumulation of inflammatory mediators and MMPs.
植物提取物和/或次生代谢产物作为治疗炎症性疾病（如牙周炎）的治疗剂受到了相当多的关注，牙周炎会影响牙齿支持组织。本研究旨在探讨富含皂苷和多酚的 Grindelia robusta 提取物对 Aggregatibacter actinomycetemcomitans 脂多糖（LPS）诱导的炎症介质（IL-6、TNF-a、RANTES、MCP-1、PGE（2））和基质金属蛋白酶（MMP-1、-3、-7、-8、-9、-13）分泌的影响。通过酶联免疫吸附试验测定，LPS 诱导巨噬细胞中所有炎症介质和 MMP 的分泌明显增加。在非细胞毒性浓度下，G. robusta 提取物剂量依赖性地抑制 IL-6、RANTES、MCP-1 的分泌，并在一定程度上抑制 PGE（2）和 TNF-a 的分泌。MMP-1、-3、-7、-8、-9 和 -13 的分泌也观察到这种抑制作用。G. robusta 提取物减少 LPS 诱导的炎症介质和 MMP 分泌的能力与核因子-kappa B（NF-kB）p65 激活的减少有关。研究结果表明，G. robusta 提取物通过减少炎症介质和 MMP 的积累，具有抗炎治疗潜力。
