Neutrophil Immunomodulatory Activity of (-)-Borneol, a Major Component of Essential Oils Extracted from .

(Pursh) Dunal is used in traditional medicine for treating various diseases; however, little is known about the immunomodulatory activity of essential oils from this plant. Thus, we isolated essential oils from the flowers (GEO) and leaves (GEO) of and evaluated the chemical composition and innate immunomodulatory activity of these essential oils. Compositional analysis of these essential oils revealed that the main components were α-pinene (24.7 and 23.2% in GEO and GEO, respectively), limonene (10.0 and 14.7%), borneol (23.4 and 16.6%), -cymen-8-ol (6.1 and 5.8%), β-pinene (4.0 and 3.8%), bornyl acetate (3.0 and 5.1%), -pinocarveol (4.2 and 3.7%), spathulenol (3.0 and 2.0%), myrtenol (2.5 and 1.7%), and terpinolene (1.7 and 2.0%). Enantiomer analysis showed that α-pinene, β-pinene, and borneol were present primarily as (-)-enantiomers (100% enantiomeric excess (ee) for (-)-α-pinene and (-)-borneol in both GEO and GEO; 82 and 78% ee for (-)-β-pinene in GEO and GEO), while limonene was present primarily as the (+)-enantiomer (94 and 96 ee in GEO and GEO). essential oils activated human neutrophils, resulting in increased [Ca] (EC = 22.3 µg/mL for GEO and 19.4 µg/mL for GEO). In addition, one of the major enantiomeric components, (-)-borneol, activated human neutrophil [Ca] (EC = 28.7 ± 2.6), whereas (+)-borneol was inactive. Since these treatments activated neutrophils, we also evaluated if they were able to down-regulate neutrophil responses to subsequent agonist activation and found that treatment with essential oils inhibited activation of these cells by the -formyl peptide receptor 1 (FPR1) agonist MLF and the FPR2 agonist WKYMVM. Likewise, (-)-borneol inhibited FPR-agonist-induced Ca influx in neutrophils. leaf and flower essential oils, as well as (-)-borneol, also inhibited MLF-induced chemotaxis of human neutrophils (IC = 4.1 ± 0.8 µg/mL, 5.0 ± 1.6 µg/mL, and 5.8 ± 1.4 µM, respectively). Thus, we identified (-)-borneol as a novel modulator of human neutrophil function.