Norioka K, Hara M, Harigai M, Kitani A, Hirose T, Hirose W, Suzuki K, Kawakami M, Kawagoe M, Nakamura H
First Department of Medicine, National Defense Medical College, Tokorozawa, Japan.
Autoimmunity. 1990;7(1):41-50. doi: 10.3109/08916939009041049.
Systemic vasculitis is an inflammatory disorder of blood vessels characterized by a perivascular mononuclear cell infiltration around the vessel and fibrinoid necrosis within vessel walls. Interleukin-1 (IL-1) is a multipotent inflammatory mediator and affects several properties of vascular cells. To determine whether IL-1 could contribute to the pathogenesis of vascular diseases, we examined the effect of IL-1 on B cell stimulatory factor-2/interleukin-6 (IL-6) production by cultured human vascular smooth muscle cells (SMC) and the proliferation of these cells. Supernatants of SMC stimulated IgM synthesis of human B cell line. SKW6-CL4 cells. This activity was increased (1.7 to 2.6-fold) when SMC were pretreated with IL-1 or calcium ionophore A23187 for 48 h, and was completely blocked by rabbit anti-human IL-6 antibodies. These IL-6 activities of the SMC supernatants were also assessed by using an IL-6 dependent murine hybridoma cell line. MH-60. BSF-2. In addition, we observed that pretreatment of SMC with IL-1 for 48 h stimulated growth of SMC during the 96 h incubations, as assessed by cell number (p less than 0.05). These results suggest that IL-1 may contribute to the pathogenesis of inflammatory and immunological vasculitis by the augmentation of IL-6 release and growth of SMC.
系统性血管炎是一种血管炎症性疾病，其特征为血管周围单核细胞浸润以及血管壁内纤维蛋白样坏死。白细胞介素-1（IL-1）是一种多效性炎症介质，可影响血管细胞的多种特性。为了确定IL-1是否参与血管疾病的发病机制，我们研究了IL-1对培养的人血管平滑肌细胞（SMC）产生B细胞刺激因子-2/白细胞介素-6（IL-6）的影响以及这些细胞的增殖情况。SMC的上清液可刺激人B细胞系SKW6-CL4细胞的IgM合成。当SMC用IL-1或钙离子载体A23187预处理48小时后，这种活性增加了（1.7至2.6倍），并且被兔抗人IL-6抗体完全阻断。SMC上清液的这些IL-6活性也通过使用依赖IL-6的小鼠杂交瘤细胞系MH-60.BSF-2进行评估。此外，我们观察到，用IL-1预处理SMC 48小时可在96小时培养期间刺激SMC生长，通过细胞数量评估（p小于0.05）。这些结果表明，IL-1可能通过增加IL-6释放和SMC生长而参与炎症性和免疫性血管炎的发病机制。
