Zhang Jianxin, Meng Pinjia, Zhang Daming, Zhang Wenfang, Chen Cunyi
Drug Intelligence and Forensic Center of the Ministry of Public Security, Beijing 100193, China.
Wei Sheng Yan Jiu. 2010 Sep;39(5):593-6.
To evaluate the effects of washing process on hair samples from heroin addicts, and compare the extracting method of enzymatic digestion with homogenization of hair samples.
After washing samples for 3.15 h, a mild enzymatic digestion procedure at pH 6.6 and a buffer incubating procedure in water bath at 48 degrees C during 18h was used. The samples were pre-treated by solid phase extraction using mixed mode sorbent columns (MCX Oasis). Quantitative analysis of morphine, codeine, 6-acetylmorphine, heroin, acetylcodeine by chromatography-tandem mass spectrometry method was developed.
Heroin accounted for 21.82% of the total equivalents of morphine. The ratio of morphine to 6-MAM in enzymatic digestion hair samples was 0.9875 and that in homogenized hair samples was 0.3948.
The results suggested that the procedures were not sufficient to remove the contaminants penetrating into hair from external sources.
评估清洗过程对海洛因成瘾者毛发样本的影响，并比较毛发样本酶消化提取方法与匀浆法。
样本清洗3.15小时后，采用pH值为6.6的温和酶消化程序以及在48℃水浴中进行18小时的缓冲液孵育程序。样本通过使用混合型吸附柱（MCX Oasis）的固相萃取进行预处理。建立了通过色谱-串联质谱法对吗啡、可待因、6-乙酰吗啡、海洛因、乙酰可待因进行定量分析的方法。
海洛因占吗啡总当量的21.82%。酶消化毛发样本中吗啡与6-单乙酰吗啡的比例为0.9875，匀浆毛发样本中该比例为0.3948。
结果表明这些程序不足以去除从外部渗入毛发的污染物。
