Department of Phytopathology, University of Life Sciences Lublin, Poland.
Pol J Microbiol. 2010;59(3):191-200.
Colletotrichum dematium was isolated from caraway for the first time in Poland in 2005. Isolations of this fungus were repeated in 2006 and 2007. The cultures of fungus were obtained from superficially disinfected leaves, root necks, roots, stems and umbels. The isolates were identified on culture media: PDA and malt agar with addition of pieces of caraway stems and on the base of macro and microscopic structures. Studies on the biotic effect between C. dematium and other species of phyllosphere fungi of caraway showed that the majority of the studied species limited the growth and development of C. dematium, but the size of the limiting effect was different. The species from Trichoderma and Gliocladium genera were the most effective against C. dematium, causing degeneration and lysis of hyphae and precluded the formation of the pathogen's acervuli and conidia. C. dematium in dual culture with E. purpurascens, A. radicina, S. sclerotiorum, B. cinerea and R. solani produced an inhibition zone which indicated its capacity for antibiosis.
2005 年,波兰首次从葛缕子中分离出了炭疽菌。2006 年和 2007 年又重复了该真菌的分离。真菌培养物是从表面消毒的叶片、根茎、根、茎和伞形花序中获得的。这些分离物是在培养介质(PDA 和添加葛缕子茎段的麦芽琼脂)上,并根据宏观和微观结构进行鉴定的。炭疽菌与葛缕子叶际真菌其他物种之间的生物效应研究表明,大多数研究的物种限制了炭疽菌的生长和发育,但限制作用的大小不同。木霉属和拟青霉属的物种对炭疽菌的抑制作用最为有效,导致菌丝变性和裂解,并阻止病原菌分生孢子头和分生孢子的形成。炭疽菌与 E. purpurascens、A. radicina、S. sclerotiorum、B. cinerea 和 R. solani 进行双重培养时,会产生一个抑制区,表明其具有抑菌能力。