CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety National Center for NanoScience and NanoTechnology, Beijing, China 100190.
Langmuir. 2010 Dec 7;26(23):17790-4. doi: 10.1021/la103132m. Epub 2010 Nov 1.
This report establishes an in vitro model on glass surfaces for patterning multiple types of cells to simulate cell-cell interactions in vivo. The model employs a microfluidic system and poly(ethylene glycol)-terminated oxysilane (PEG-oxysilane) to modify glass surfaces in order to resist cell adhesion. The system allows the selective confinement of different types of cells to realize complete confinement, partial confinement, and no confinement of three types of cells on glass surfaces. The model was applied to study intercellular interactions among human umbilical vein endothelial cells (HUVEC), PLA 801 C and PLA801 D cells.
本报告在玻璃表面上建立了一种体外模型，用于对多种类型的细胞进行图案化，以模拟体内的细胞-细胞相互作用。该模型采用微流控系统和聚（乙二醇）末端的硅氧烷（PEG-oxysilane）来修饰玻璃表面，以抵抗细胞黏附。该系统允许对不同类型的细胞进行选择性限制，从而实现三种细胞在玻璃表面上的完全限制、部分限制和无限制。该模型应用于研究人脐静脉内皮细胞（HUVEC）、PLA801C 和 PLA801D 细胞之间的细胞间相互作用。
