D'Ovidio R, Tanzarella O A, Porceddu E
Dipartimento di Agrobiologia e Agrochimica, Università della Tuscia, Viterbo, Italy.
Plant Mol Biol. 1990 Jul;15(1):169-71. doi: 10.1007/BF00017737.
The polymerase chain reaction (PCR) was used to amplify genomic DNA of several wheat genotypes. The oligonucleotides used as primers were the terminal sequences of a gamma-gliadin gene. The electrophoretic analysis of the PCR products showed specific bands which revealed both inter- and intra-specific genetic polymorphism among the examined genotypes. The technique is proposed as a very simple and efficient alternative to RFLP markers.
聚合酶链反应（PCR）用于扩增几种小麦基因型的基因组DNA。用作引物的寡核苷酸是γ-醇溶蛋白基因的末端序列。PCR产物的电泳分析显示出特异性条带，揭示了所检测基因型之间的种间和种内遗传多态性。该技术被认为是一种非常简单有效的限制性片段长度多态性（RFLP）标记替代方法。
