Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Nobelsv. 16, Stockholm, Sweden.
Semin Cancer Biol. 2010 Dec;20(6):410-5. doi: 10.1016/j.semcancer.2010.10.013. Epub 2010 Oct 27.
CLL is not associated with EBV. CLL cells separated from blood express CR2, the complement receptor that serves also as EBV receptor. Thus CLL cells can be infected in vitro with the virus, however, in contrast to normal B lymphocytes, only rare CLL clones yield transformed lines. This is due to a restricted EBV encoded protein expression in the CLL cells, they express EBNAs, the virus encoded proteins that are localized in the nucleus, but not the cell membrane associated LMP-1, that is also pivotal for the virus induced transformation of B lymphocytes. This expression pattern seems to be unique to a defined B cell maturation window that is represented by the CLL cells. We named this restricted viral expression as Type IIb. Such B lymphocytes have been encountered in lymphoid tissues of infectious mononucleosis (IM) and in post transplant lymphoproliferative disease (PTLD). Moreover, they were shown in tissues of EBV infected "humanized" mice. The EBV encoded protein expression pattern may serve as a marker for the B cell differentiation stage from which CLL clones can develop.
CLL 与 EBV 无关。从血液中分离出来的 CLL 细胞表达 CR2，即补体受体，也可作为 EBV 受体。因此，CLL 细胞可以在体外被病毒感染，然而，与正常 B 淋巴细胞不同，只有极少数的 CLL 克隆产生转化系。这是由于 CLL 细胞中 EBV 编码蛋白的表达受到限制，它们表达 EBNAs，即病毒编码的蛋白，定位于细胞核内，但不表达细胞膜相关的 LMP-1，后者对于 B 淋巴细胞的病毒诱导转化也至关重要。这种表达模式似乎是由 CLL 细胞代表的特定 B 细胞成熟窗口所特有的。我们将这种受限的病毒表达称为 IIb 型。在传染性单核细胞增多症 (IM) 的淋巴组织和移植后淋巴组织增生性疾病 (PTLD) 中也遇到过这种具有受限病毒表达的 B 淋巴细胞。此外，在 EBV 感染的“人源化”小鼠的组织中也观察到了它们的存在。EBV 编码蛋白的表达模式可作为 CLL 克隆可能发育的 B 细胞分化阶段的标志物。
