Institute of Pathology-ICMR, Safdarjung Hospital Campus, New Delhi, India.
Microbes Infect. 2011 Feb;13(2):167-78. doi: 10.1016/j.micinf.2010.10.012. Epub 2010 Oct 27.
To investigate whether IL-17A (IL-17) and IL-22 are produced in response to Chlamydia trachomatis infection, the cervical washes of 27 women with C. trachomatis infection and 17 C. trachomatis negative controls were collected. The levels of cytokines were determined in the cervical wash and in the supernatant of cervical and systemic cell cultures upon C. trachomatis antigen stimulation. C. trachomatis infection appeared to activate local IL-17 and IL-22 production more efficiently than IFN-γ production. In the cervical wash of infected women, median concentrations of IL-17 and -22 were 5- and 3-fold higher, respectively, than in negative controls. The spontaneous intracellular expression of these cytokines was analysed by flow cytometry in blood and cervical cells and 26% of cervical mononuclear cells from infected women were shown to produce IL-22 and 12% to coproduce IL-17 and IL-22. In addition, it was demonstrated that 20-25% of IL-22 producing and IL-17-IL-22 coproducing cervical CD4+ T cells expressed the mucosal homing receptor CCR6. These results suggest that CCR6 is involved in the migration of these cells to the cervix and that IL-17 and IL-22 might play a role in the immune response at the site of C. trachomatis infection.
为了研究白细胞介素 17A（IL-17）和白细胞介素 22（IL-22）是否是针对沙眼衣原体（Chlamydia trachomatis）感染而产生的，收集了 27 名沙眼衣原体感染女性和 17 名沙眼衣原体阴性对照者的宫颈洗液。在沙眼衣原体抗原刺激下，测定宫颈洗液和宫颈及全身细胞培养上清液中的细胞因子水平。沙眼衣原体感染似乎比 IFN-γ 更有效地激活局部 IL-17 和 IL-22 的产生。在感染女性的宫颈洗液中，IL-17 和-22 的中位数浓度分别比阴性对照组高 5 倍和 3 倍。通过流式细胞术分析血液和宫颈细胞中这些细胞因子的自发细胞内表达，结果显示 26%的感染女性宫颈单核细胞产生 IL-22，12%的宫颈单核细胞共产生 IL-17 和 IL-22。此外，还证明 20-25%产生 IL-22 的和共产生 IL-17 和 IL-22 的宫颈 CD4+T 细胞表达黏膜归巢受体 CCR6。这些结果表明 CCR6 参与这些细胞向宫颈的迁移，IL-17 和 IL-22 可能在沙眼衣原体感染部位的免疫反应中发挥作用。
