Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, Av San Rafael Atlixco No 186, Col Vicentina, Del Iztapalapa, México DF CP 09340, Mexico.
J Hazard Mater. 2011 Jan 30;185(2-3):1592-5. doi: 10.1016/j.jhazmat.2010.09.100. Epub 2010 Oct 28.
The kinetic behavior of a nitrifying sludge exposed to 2-chlorophenol (2-CP) was evaluated in batch culture. The assays were performed using a stabilized nitrifying sludge. In control assays with (mg L(-1)): NH(4)(+)-N (100) and NaHCO(3)(-)-C (250), the substrates were consumed in 8h, the ammonium consumption efficiency was 99% and the NO(3)(-) yield higher than 0.9. When 5mg 2-CP-C L(-1) was added, it was transformed into an unidentified intermediate and the nitrifying efficiency decreased to 10%. Ammonium specific consumption rate diminished 95%, but the NO(3)(-) yield remained higher than 0.9. The biomass previously exposed to 2-CP was newly suspended with NH(4)(+)-N or NO(2)(-)-N in order to evaluate the ammonium and nitrite oxidizing processes. The consumption efficiencies and NO(3)(-) yields were similar to those obtained in control assays. However, the total time required for ammonium and nitrite consumption increased to 120 and 42 h, respectively. Specific consumption rates for NH(4)(+)-N and NO(2)(-)-N decreased by 95% and 83% respectively, compared to control assays. Thus, the previous contact to 2-CP had more influence on ammonium oxidizing process than the nitrite oxidizing process. These are the first evidences where a nitrifying sludge exposed to 2-CP are reported.
在批处理培养中评估了硝化污泥暴露于 2-氯苯酚（2-CP）时的动力学行为。使用稳定的硝化污泥进行了测定。在对照实验中（mg L(-1))：NH(4)(+)-N (100)和 NaHCO(3)(-)-C (250)，底物在 8 小时内被消耗，铵盐消耗效率为 99%，NO(3)(-)产率高于 0.9。当添加 5mg 2-CP-C L(-1)时，它被转化为一种未知的中间产物，硝化效率降低到 10%。铵盐比消耗速率降低了 95%，但 NO(3)(-)产率仍高于 0.9。先前暴露于 2-CP 的生物质与 NH(4)(+)-N 或 NO(2)(-)-N 重新悬浮，以评估铵盐和亚硝酸盐氧化过程。消耗效率和 NO(3)(-)产率与对照实验相似。然而，铵盐和亚硝酸盐消耗所需的总时间分别增加到 120 和 42 小时。与对照实验相比，NH(4)(+)-N 和 NO(2)(-)-N 的比消耗速率分别降低了 95%和 83%。因此，先前接触 2-CP 对铵盐氧化过程的影响比对亚硝酸盐氧化过程的影响更大。这是首次报道硝化污泥暴露于 2-CP 的情况。
