Pérez-Alfaro J E, González-Blanco G, Sierra-Palacios E, Marcial-Quino J, Beristain-Cardoso R
Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, México, D.F.
Academia de Biología, Colegio de Ciencias y Humanidades, Universidad Autónoma de la Ciudad de México, México.
Water Sci Technol. 2015;71(2):277-82. doi: 10.2166/wst.2014.508.
The metabolic and kinetic behavior of a nitrifying sludge exposed to 2-chlorophenol (2-CP) was evaluated in batch cultures. Two kinds of nitrifying culture were used; one acclimated to 4-methylphenol (4-mp), and the other unacclimated to 4-mp. The unacclimated culture was affected adversely by the 2-CP's presence, since neither nitrification nor 2-CP oxidation was observed. Nonetheless, the acclimated culture showed metabolic capacity to nitrify and mineralize 2-CP. Ammonium removal was 100%, with a nitrifying yield of 0.92 ± 0.04 mg NO(3)(-)-N/mg NH(4)(+)-N consumed. The consumption efficiency for 2-CP was 100% and the halogenated compound was mineralized to CO2. Denaturing gradient gel electrophoresis (DGGE) patterns showed the shift in microbial community structure, indicating that microbial diversity was due to the acclimation process. This is the first evidence where nitrifying culture acclimated to 4-mp completely removed ammonium and 2-CP.
在分批培养中评估了暴露于2-氯苯酚（2-CP）的硝化污泥的代谢和动力学行为。使用了两种硝化培养物；一种适应4-甲基苯酚（4-mp），另一种未适应4-mp。未适应的培养物受到2-CP存在的不利影响，因为未观察到硝化作用和2-CP氧化。尽管如此，适应的培养物显示出硝化和矿化2-CP的代谢能力。铵去除率为100%，硝化产率为0.92±0.04 mg NO(3)(-)-N/mg消耗的NH(4)(+)-N。2-CP的消耗效率为100%，卤代化合物矿化为CO2。变性梯度凝胶电泳（DGGE）图谱显示微生物群落结构发生了变化，表明微生物多样性是由于适应过程所致。这是首次有证据表明适应4-mp的硝化培养物能完全去除铵和2-CP。
