Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
Phytomedicine. 2010 Dec 15;18(1):36-9. doi: 10.1016/j.phymed.2010.09.005. Epub 2010 Oct 28.
Parthenolide previously isolated from Tanacetum vulgare was tested for its in vitro combinatory effect with benznidazole against Trypanosoma cruzi. Parthenolide showed a strong synergistic activity against epimastigote forms, reducing 23-fold the concentration of benznidazole necessary to inhibit 50% of cell growth (IC(50) of 1.6 to 0.07 μg/ml) when in combination with parthenolide. In addition, an additive effect against trypomastigote forms (FIC 1.06), followed by an antagonistic effect on the cytotoxicity (FIC 2.36), was observed for the combination of both drugs. Parthenolide induced morphological alterations in the body shape of trypomastigote forms, causing rounding and shortening of the parasite and loss of integrity of the plasma membrane, as previously described by other workers.
此前从菊科植物春黄菊中分离得到的小白菊内酯与苯并咪唑联合测试了对克氏锥虫的体外协同作用。小白菊内酯对滋养体形式表现出强烈的协同活性，当与小白菊内酯联合使用时，使抑制细胞生长 50%所需的苯并咪唑浓度降低 23 倍（IC50 为 1.6 至 0.07μg/ml）。此外，还观察到联合使用这两种药物对锥虫形式具有相加作用（FIC 1.06），随后对细胞毒性具有拮抗作用（FIC 2.36）。如其他研究人员所述，小白菊内酯诱导锥虫滋养体形态的形状发生改变，导致寄生虫变圆和缩短，以及质膜完整性丧失。
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