Métaux et Microorganismes: Chimie, Biologie et Applications, FRE 3211, ESBS, Strasbourg, France.
FEBS Lett. 2010 Dec 1;584(23):4751-5. doi: 10.1016/j.febslet.2010.10.051. Epub 2010 Oct 28.
Pseudomonas aeruginosa secretes the fluorescent siderophore, pyoverdine (PVD), to enable iron acquisition. Epifluorescence microscopy and cellular fractionation were used to investigate the role of an efflux pump, PvdRT-OpmQ, in PVD secretion. Bacteria lacking this efflux pump accumulated PVD, or a fluorescent precursor, in the periplasm, due to their inability to efficiently secrete into the media newly synthesized PVD. PvdRT-OpmQ is only the second system identified for secretion of newly synthesized siderophores by Gram negative bacteria.
铜绿假单胞菌分泌荧光铁载体，即绿脓菌素 (PVD)，以实现铁的摄取。使用荧光显微镜和细胞分级分离来研究外排泵 PvdRT-OpmQ 在 PVD 分泌中的作用。由于缺乏这种外排泵的细菌无法有效地将新合成的 PVD 分泌到培养基中，因此它们在周质中积累 PVD 或荧光前体。PvdRT-OpmQ 是革兰氏阴性菌中鉴定出的第二个用于分泌新合成的铁载体的系统。
