Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Beijing 100050, People's Republic of China.
Mech Ageing Dev. 2010 Nov-Dec;131(11-12):723-31. doi: 10.1016/j.mad.2010.10.003. Epub 2010 Oct 28.
Although salidroside and salidroside-like compounds are considered as most critical constitutes needed and responsible for multiple therapeutic benefits of Rhodiola rosea L., including anti-aging, direct demonstration regarding the role of salidroside in anti-aging process is still deficient. In this study, we selected the H(2)O(2)-induced premature senescence model in human fetal lung diploid fibroblasts to investigate the protection of salidroside against aging in vitro and associated molecular mechanisms. We found that salidroside considerably reversed senescence-like phenotypes in the oxidant challenged model, including alterations of morphology, cell cycle, SA-β-gal staining, DNA damage, as well as related molecules expression such as p53, p21 and p16. The protection occurred in a dose-dependent manner, with 5μM offering best efficacy. The proposed antioxidant property of the compound was confirmed in this cellular system, and thus at least partially accounted for the protection of the compound against premature senescence. Similar protection of salidroside against replicative senescence was observed as well. Interestingly, the regulation of senescence-related molecules by salidroside involved ROS-irrelevant mechanisms in both models. This finding presents salidroside as an attractive agent with potential to retard aging and attenuate age-related diseases in humans.
虽然红景天苷和红景天苷类似化合物被认为是红景天发挥多种治疗功效（包括抗衰老）所必需的关键成分，但直接证明红景天苷在抗衰老过程中的作用仍然不足。在这项研究中，我们选择 H 2 O 2 诱导的人胚肺二倍体成纤维细胞衰老模型，以研究红景天苷对体外衰老的保护作用及其相关分子机制。我们发现，红景天苷能显著逆转氧化应激模型中的衰老样表型，包括形态改变、细胞周期停滞、SA-β-半乳糖苷染色、DNA 损伤以及 p53、p21 和 p16 等相关分子的表达。这种保护作用呈剂量依赖性，5μM 时效果最佳。该化合物在该细胞体系中的抗氧化特性得到了证实，因此至少部分解释了该化合物对早期衰老的保护作用。红景天苷对复制性衰老的保护作用也得到了观察。有趣的是，在两种模型中，红景天苷对衰老相关分子的调节涉及与 ROS 无关的机制。这一发现表明红景天苷作为一种有吸引力的抗衰老药物，具有延缓衰老和减轻与年龄相关疾病的潜力。
