Tierney Keith B
Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada.
Biochim Biophys Acta. 2011 Mar;1812(3):381-9. doi: 10.1016/j.bbadis.2010.10.011. Epub 2010 Oct 28.
Altered neurological function will generally be behaviourally apparent. Many of the behavioural models pioneered in mammalian models are portable to zebrafish. Tests are available to capture alterations in basic motor function, changes associated with exteroceptive and interoceptive sensory cues, and alterations in learning and memory performance. Excepting some endpoints involving learning, behavioural tests can be carried out at 4 days post fertilization. Given larvae can be reared quickly and in large numbers, and that software solutions are readily available from multiple vendors to automatically test behavioural responses in 96 larvae simultaneously, zebrafish are a potent and rapid model for screening neurological impairments. Coupling current and emerging behavioural endpoints with molecular techniques will permit and accelerate the determination of the mechanisms behind neurotoxicity and degeneration, as well as provide numerous means to test remedial drugs and other therapies. The emphasis of this review is to highlight unexplored/underutilized behavioural assays for future studies. This article is part of a Special Issue entitled Zebrafish Models of Neurological Diseases.
神经功能改变通常会在行为上表现出来。许多在哺乳动物模型中开创的行为模型可应用于斑马鱼。现有测试可用于捕捉基本运动功能的改变、与外感受和内感受感觉线索相关的变化以及学习和记忆表现的改变。除了一些涉及学习的终点指标外，行为测试可在受精后4天进行。鉴于斑马鱼幼体能够快速大量饲养，并且有多个供应商提供的软件解决方案可同时自动测试96条幼体的行为反应，斑马鱼是一种高效且快速的神经损伤筛查模型。将当前和新出现的行为终点指标与分子技术相结合，将有助于并加速确定神经毒性和神经变性背后的机制，同时提供多种测试补救药物和其他疗法的方法。本综述的重点是突出未被探索/未充分利用的行为分析方法以供未来研究。本文是名为《神经疾病的斑马鱼模型》的特刊的一部分。
