Analytical Division, Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
Anal Chim Acta. 2010 Nov 29;681(1-2):16-26. doi: 10.1016/j.aca.2010.09.027. Epub 2010 Sep 24.
The present work describes a new, simple, and easy method for the generation of stable molecularly imprinted sites in polymeric film, combining self-assembled monolayer and Layer-by-layer approaches through thermal cross-linking of the layered structures, onto the surface of silver electrode. Modified silver electrodes demonstrate enantiodifferentiation and sensitive (detection limits 0.0060 ng mL(-1) for L- and 0.0062 for D-thyroxine) determination of d- and l-thyroxine with the help of differential pulse anodic stripping voltammetric technique. The binding kinetics of thyroxine was explored using anodic stripping cyclic voltammetry and chronocoulometry. The sensor was also validated for D- and L-thyroxine determinations in biological and pharmaceutical samples.
本工作描述了一种新的、简单且易于应用的方法，可在银电极表面上通过层状结构的热交联，将自组装单分子层和层层方法结合起来，在聚合膜中生成稳定的分子印迹点。修饰后的银电极通过差分脉冲阳极溶出伏安技术，对 d-和 l-甲状腺素进行了对映体选择性和灵敏（检测限：L-甲状腺素为 0.0060ng mL(-1)，D-甲状腺素为 0.0062ng mL(-1)）的测定。使用阳极溶出循环伏安法和计时库仑法研究了甲状腺素的结合动力学。该传感器还用于生物和药物样品中 D-和 L-甲状腺素的测定。
