Analytical Division, Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
Talanta. 2010 Apr 15;81(1-2):187-96. doi: 10.1016/j.talanta.2009.11.055. Epub 2009 Nov 29.
Electrochemical sensors demonstrating enantioselectivity to tryptophan enantiomers, with high selectivity and sensitivity, were fabricated by the use of a monolithic fiber of molecularly imprinted polymer-carbon composite. The recognition mechanism and performance of these sensors were evaluated by differential pulse anodic stripping voltammetry. The sensor imprinted for l-tryptophan not only discriminated the target from its analogues and other amino acids but also responded specifically in racemic mixture in aqueous, biological, and pharmaceutical samples. The binding kinetics of L-tryptophan was also established with the help of anodic stripping cyclic voltammetry and chronocoulometry. The detection limit for L-tryptophan was as low as 0.24 ng mL(-1) (signal/noise=3) which is appropriate for biomarking diseases, caused by an acute tryptophan-depletion, in clinical setting.
电化学传感器通过使用分子印迹聚合物-碳复合材料整体纤维展示了对色氨酸对映体的对映选择性，具有高选择性和灵敏度。通过差分脉冲阳极溶出伏安法评估了这些传感器的识别机制和性能。对 l-色氨酸进行印迹的传感器不仅可以将目标物与其类似物和其他氨基酸区分开来，而且可以在水相、生物和药物样品的外消旋混合物中特异性响应。借助阳极溶出循环伏安法和计时库仑法还建立了 l-色氨酸的结合动力学。l-色氨酸的检测限低至 0.24ngmL(-1)（信号/噪声=3），适用于临床环境中由急性色氨酸耗竭引起的疾病的生物标志物检测。
