Departamento de Química, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Agencia Postal N° 3, 5800-Río Cuarto, Córdoba, Argentina.
Talanta. 2010 Nov 15;83(1):249-55. doi: 10.1016/j.talanta.2010.09.016. Epub 2010 Sep 19.
An amperometric biosensor based on peroxidases from Brassica napus hairy roots (PBHR) used to determine the total polyphenolic content in wine and tea samples is proposed by the first time. The method employs carbon paste (CP) electrodes filled up with PBHR, ferrocene (Fc), and multi-walled carbon nanotubes embedded in a mineral oil (MWCNT+MO) at a given composition (PBHR-Fc-MWCNT+MO). The biosensor was covered externally with a dialysis membrane, which was fixed at the electrode body side part with a Teflon laboratory film and an O-ring. Calibration curves obtained from steady-state currents as a function of the concentration of a polyphenolic standard reference compound such as t-resveratrol (t-Res) or caffeic acid (CA) were then used to estimate the total polyphenolic content in real samples. The reproducibility and the repeatability were of 7.0% and 4.1% for t-Res (8.4% and 5.2% for CA), respectively, showing a good biosensor performance. The calibration curves were linear in a concentration range from 0.05 to 52 mg L(-1) and 0.06 to 69 mg L(-1) for t-Res and CA, respectively. The lowest polyphenolic compound concentration values measured experimentally for a signal to noise ratio of 3:1 were 0.023 mg L(-1) and 0.020 mg L(-1) for t-Res and CA, respectively.
首次提出了一种基于油菜毛状根过氧化物酶（PBHR）的安培生物传感器，用于测定葡萄酒和茶样中的总多酚含量。该方法采用填充有 PBHR、二茂铁（Fc）和嵌入矿物油中的多壁碳纳米管（MWCNT+MO）的碳糊（CP）电极（PBHR-Fc-MWCNT+MO）。生物传感器外部覆盖有透析膜，该膜通过特氟龙实验室膜和 O 形圈固定在电极体侧部。然后，根据稳态电流与多酚标准参考化合物（如反式白藜芦醇（t-Res）或咖啡酸（CA））浓度的关系获得校准曲线，以估算实际样品中的总多酚含量。t-Res 的重现性和重复性分别为 7.0%和 4.1%（CA 的重现性和重复性分别为 8.4%和 5.2%），表明生物传感器性能良好。校准曲线在 0.05 至 52 mg L(-1) 和 0.06 至 69 mg L(-1)范围内对于 t-Res 和 CA 分别呈线性。实验测量的最低多酚化合物浓度值为 0.023 mg L(-1)和 0.020 mg L(-1)，对于 t-Res 和 CA，信噪比分别为 3:1。
