Pospísil J, Postupa J, Otcenásek M, Vejbora O
Institute of Experimental Biopharmacy, Czechoslovak Academy of Sciences, Hradec Králové.
Mycoses. 1990 Nov-Dec;33(11-12):559-66. doi: 10.1111/myc.1990.33.11-12.559.
The authors describe the course of experimental trichosporosis in normal and X-irradiated ICR mice after i.v. inoculation with Trichosporon capitatum. The irradiated animals were considerably more sensitive to infection than normal animals. The LD50 challenge dose used was by approximately two orders lower (1 X 10(3) c.f.u. ml-1) in irradiated mice than in control animals. The histopathological examination of the internal organs of the infected mice demonstrated that the greatest tissue damage was associated with the kidneys, liver and spleen. However, the infectious agent was also found in heart, lungs and brain. The degree of impairment of the tissues was dependent on the inoculation dose and on the irradiation status. Miconazole (50 mg kg-1) was administered i.p. immediately after inoculation with Ts. capitatum and resulted in an alteration of infection and prolonged survival time. Miconazole was ineffective when challenge dose were used which produced 100% mortality (1 X 10(6) and 1 X 10(4) c.f.u. ml-1 for normal and irradiated mice, respectively). With the use of these doses also the course of infection was nearly identical both in the miconazole-treated and untreated animals.
作者描述了经静脉接种头状毛孢子菌后，正常和经X射线照射的ICR小鼠实验性毛孢子菌病的病程。与正常动物相比，受照射动物对感染的敏感性要高得多。所使用的半数致死挑战剂量在受照射小鼠中（1×10³ c.f.u. ml⁻¹）比对照动物低约两个数量级。对感染小鼠内脏器官的组织病理学检查表明，最大的组织损伤与肾脏、肝脏和脾脏有关。然而，在心脏、肺和脑中也发现了感染病原体。组织损伤的程度取决于接种剂量和照射状态。接种头状毛孢子菌后立即腹腔注射咪康唑（50 mg kg⁻¹），可导致感染改变并延长存活时间。当使用导致100%死亡率的挑战剂量时（正常和受照射小鼠分别为1×10⁶和1×10⁴ c.f.u. ml⁻¹），咪康唑无效。使用这些剂量时，咪康唑治疗组和未治疗组动物的感染病程几乎相同。
