Behrens-Baumann W, Klinge B, Uter W
Department of Ophthalmology, University of Göttingen, Germany.
Mycoses. 1990 Nov-Dec;33(11-12):567-73. doi: 10.1111/myc.1990.33.11-12.567.
Using a reproducible model of Candida albicans keratitis in rabbits we studied the effect of topical clotrimazole and bifonazole. Candida albicans DSM 70010 (2.5 X 10(5) cells) was injected into the corneal stroma of both eyes of 28 rabbits. All eyes developed a corneal ulcer. Fourty-eight hours after inoculation the animals were divided into four groups: I (14 eyes) receiving 10 X clotrimazole 1% drops and subsequently removing the epithelium; II (14 eyes) receiving only clotrimazole drops; III (8 eyes) receiving 6 x bifonazole 1% drops and IV (19 eyes) serving as control (0.9% NaCl castor oil, untreated), 6 eyes of this group were also debrided. A further 6 rabbits were used respectively to judge if the drugs penetrated into the cornea and aqueous humor. There was a significant difference between the clotrimazole group with debridement (I) and the bifonazole group (IV) concerning hypopyon and complications (descemetocele, corneal perforation). Clotrimazole penetrated into the cornea and after debridement into the aqueous humor. Bifonazole could not be identified in the cornea or aqueous humor.
我们使用兔白色念珠菌角膜炎的可重复模型，研究了局部应用克霉唑和联苯苄唑的效果。将白色念珠菌DSM 70010（2.5×10⁵个细胞）注入28只兔双眼的角膜基质。所有眼睛均出现角膜溃疡。接种48小时后，将动物分为四组：I组（14只眼）接受1%克霉唑滴眼液10倍剂量并随后去除上皮；II组（14只眼）仅接受克霉唑滴眼液；III组（8只眼）接受1%联苯苄唑滴眼液6倍剂量；IV组（19只眼）作为对照组（0.9%氯化钠蓖麻油，未治疗），该组6只眼也进行了清创。另外6只兔分别用于判断药物是否渗透到角膜和房水中。在有清创的克霉唑组（I）和联苯苄唑组（IV）之间，关于前房积脓和并发症（后弹力层膨出、角膜穿孔）存在显著差异。克霉唑渗透到角膜中，清创后渗透到房水中。在角膜或房水中未检测到联苯苄唑。
