Hassan Heba A, Geniady Mahmoud M, Abdelwahab Sayed F, Abd-Elghany Manal I, Sarhan Hatem A, Abdelghany Ahmed A, Kamel Mohamed S, Rodriguez Alejandra E, Alio Jorge L
Department of Pharmacognosy, Faculty of Pharmacy, Deraya University, Minia, Egypt.
Ophthalmology Department, Faculty of Medicine, Minia University, Minia, Egypt.
Ophthalmic Res. 2018;60(2):69-79. doi: 10.1159/000488907. Epub 2018 Jul 3.
To determine the efficacy of eugenol for the treatment of Candida keratitis in an experimental model.
The in vitro antifungal activity of eugenol and fluconazole was tested against C. albicans strains via the microbroth dilution method. An experimental model of Candida albicans keratitis was used. Rabbits were classified into those that received no treatment (control; group 1) and those that started eugenol treatment immediately (group 2) or after 4 days (group 3) of keratitis induction (n = 12-16 rabbits/group). The 2 treatment groups were assigned to 50 μL of 4 mg/mL eugenol drops hourly for 15 days, while the control group received saline. Corneal penetration of eugenol was measured using HPLC, and corneal toxicity was evaluated clinically and histopathologically.
The in vitro minimum inhibitory concentrations of eugenol and fluconazole against C. albicans were 2 and > 0.4 mg/mL, respectively. A 4-mg/mL preparation of eugenol in propylene glycol was the maximum nontoxic dose on rabbit corneas as suggested by clinical and histopathologic findings. At least 75% of all eugenol-treated eyes recovered from keratitis, with improvement in the remaining 25% of the eyes compared to controls.
Eugenol can act as a natural, safe, and effective treatment for fungal keratitis, regardless of whether treatment is started immediately or after 4 days of keratitis induction.
在实验模型中确定丁香酚治疗白色念珠菌性角膜炎的疗效。
通过微量肉汤稀释法检测丁香酚和氟康唑对白色念珠菌菌株的体外抗真菌活性。使用白色念珠菌性角膜炎的实验模型。将兔子分为未接受治疗的组（对照组；第1组）和在角膜炎诱导后立即（第2组）或4天后（第3组）开始丁香酚治疗的组（每组12 - 16只兔子）。两个治疗组每小时滴入50 μL 4 mg/mL的丁香酚滴眼液，持续15天，而对照组接受生理盐水。使用高效液相色谱法测量丁香酚的角膜渗透率，并通过临床和组织病理学评估角膜毒性。
丁香酚和氟康唑对白色念珠菌的体外最低抑菌浓度分别为2和> 0.4 mg/mL。临床和组织病理学结果表明，丙二醇中4 mg/mL的丁香酚制剂是兔子角膜的最大无毒剂量。所有接受丁香酚治疗的眼睛中至少75%从角膜炎中恢复，与对照组相比，其余25%的眼睛有所改善。
无论在角膜炎诱导后立即开始治疗还是4天后开始治疗，丁香酚都可作为真菌性角膜炎的天然、安全且有效的治疗方法。
