State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, PR China.
Biosens Bioelectron. 2011 Jan 15;26(5):2329-33. doi: 10.1016/j.bios.2010.10.005. Epub 2010 Oct 14.
In present work, a rhodamine 6G (Rh 6G)-incorporated β-cyclodextrin functionalized gold nanoparticle (Rh 6G-CD-AuNP) based fluorescent assay has been successfully developed for recognizing/detecting the structural isomers, α-naphthol and β-naphthol, in aqueous solution. The β-cyclodextrin functionalized gold nanoparticles (CD-AuNPs) are achieved by conjugating the thiolated β-cyclodextrin (SH-β-CD) with AuNPs via S-Au covalent bonds. Rhodamine 6G (Rh 6G) is chosen as a fluorescent probe in this approach because it can be strongly absorbed on the surface of AuNP by noncovalent interaction. After binding with β-CD cavity, the naphthols enable to act as electron transfer quenchers of Rh 6G, which lead to significant fluorescence quenching of the dye. Because of different association ability of naphthol isomers with the β-CD cavity, the assay can selectively distinguish α-naphthol and β-naphthol with reasonable sensitivity. Detection of naphthols down to 8 nM with a dynamic range of nearly three orders of magnitude (0.01-8 μM) for α-naphthol and 50 nM with two orders of magnitude (0.1-20 μM) for β-naphthol is demonstrated, respectively. The ability of the method for detecting the content of α-naphthol or β-naphthol in the different naphthol mixtures has also been evaluated.
在目前的工作中，成功开发了一种基于罗丹明 6G（Rh 6G）结合的β-环糊精功能化金纳米粒子（Rh 6G-CD-AuNP）的荧光分析方法，用于识别/检测水溶液中的结构异构体α-萘酚和β-萘酚。β-环糊精功能化金纳米粒子（CD-AuNPs）是通过将巯基-β-环糊精（SH-β-CD）与 AuNPs 通过 S-Au 共价键连接而实现的。在这种方法中，选择罗丹明 6G（Rh 6G）作为荧光探针，因为它可以通过非共价相互作用强烈吸附在 AuNP 表面上。与β-CD 腔结合后，萘酚能够作为 Rh 6G 的电子转移猝灭剂，导致染料的荧光显著猝灭。由于萘酚异构体与β-CD 腔的不同结合能力，该测定法可以选择性地区分α-萘酚和β-萘酚，具有合理的灵敏度。分别对α-萘酚检测下限低至 8 nM，动态范围近三个数量级（0.01-8 μM），对β-萘酚检测下限低至 50 nM，动态范围两个数量级（0.1-20 μM）。该方法检测不同萘酚混合物中α-萘酚或β-萘酚含量的能力也得到了评估。
